Enhancement of plasmid DNA immunogenicity with linear polyethylenimine
نویسندگان
چکیده
منابع مشابه
Transcription of plasmid DNA: influence of plasmid DNA/polyethylenimine complex formation.
Polyethylenimine (PEI) is one of the most potent non-viral vectors. We have developed a lactosylated PEI (Lac-PEI) to enhance cell-specific transfection and have shown that Lac-PEI is more efficient than unsubstituted PEI for gene transfer into immortalized cystic fibrosis airway epithelial SigmaCFTE29o-cells. As both intact PEI/plasmid and Lac-PEI/plasmid complexes are found in the cell nucleu...
متن کاملLinear polyethylenimine-plasmid DNA nanoparticles are ototoxic to the cultured sensory epithelium of neonatal mice.
Polyethylenimine (PEI) has been demonstrated to be an effective non‑viral synthetic polymer for efficient gene delivery amongst various cell types in vitro and in vivo. In the present study, 25 kDa linear PEI (L‑PEI) was used to transfer plasmid DNA (pDNA), encoding the enhanced green fluorescent protein reporter gene, into the cultured cochlear epithelium of neonatal mice. The 25 kDa L‑PEI/pDN...
متن کاملEvaluation of proinflammatory cytokine production induced by linear and branched polyethylenimine/plasmid DNA complexes in mice.
The purpose of this study was to evaluate the cytokine response induced by linear and branched polyethylenimine (PEI)/plasmid DNA (pDNA) complex (polyplex) in relation to the ratio of PEI nitrogen and DNA phosphate (N/P ratio) of the polyplex, dose of pDNA, and structure and molecular weight of PEI, which are important for transfection efficacy of PEI polyplex. As a control, a N-[1-(2, 3-dioley...
متن کاملCrosslinked linear polyethylenimine enhances delivery of DNA to the cytoplasm.
Crosslinked polyethylenimines (PEIs) have been frequently examined over the past decade since they can maintain the transfection efficiency of commercially available, 25k branched PEI, but exhibit less cytotoxicity. The argument is often made that the degradability of such polymers, generally synthesized with either disulfide or hydrolytically degradable crosslinkers, is critical to the high ef...
متن کاملWhich mechanism for nuclear import of plasmid DNA complexed with polyethylenimine derivatives?
BACKGROUND To investigate the nuclear import mechanism of plasmid/polyethylenimine (PEI) derivative complexes and the putative nuclear targeting of therapeutic genes by the use of oligosaccharides, we have studied the nuclear import of plasmid DNA complexed either with PEI or with lactosylated PEI (Lac-PEI) in cystic fibrosis human airway epithelial cells ( summation operatorCFTE29o- cells). ...
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ژورنال
عنوان ژورنال: European Journal of Immunology
سال: 2012
ISSN: 0014-2980
DOI: 10.1002/eji.201242410